Gel-Solubilization-Buffer

Recipe I
60% Guanidine Thiocyanate (5.36M)
50mM Tris-HCl pH 7.5
0.006% Phenol Red

Use 3 Volume of buffer to 1 gel volume.

Recipe II

60% Guanidine Thiocyanate (5.36M)
140mM MES (2-[N-Morpholino] ethanesulfonic acid)
0.006% Phenol Red

Use 3 Volume of buffer to 1 gel volume.

Phenol Red is only a pH indicator. It changes color from yellow to red over the pH range 6.8 to 8.4.

Reference: http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0007750

Recipe III

6M Guanidine Thiocyante
50mM Tris-HCl pH 7.5
20mM EDTA pH 8.0

Use 3 Volume of buffer to 1 gel volume.