High-Yield-Plasmid-Medium

Low Yield LB medium (g/L):

tryptone, 10;  yeast extract, 5.0; NaCl, 10.0.

High Yield Medium (g/L):

Tryptone, 10.0; yeast extract, 10 or 15; NaCl, 10.0--> 930 ml H2O;

or 25 g LB mix powder + 10 g yeast extract --->930 ml H2O;

Trace metal solution, 1 ml/L;

50 X 2G, 20 ml;

20 X P, 50 ml;

Ampicillin, 100 mg/L,  i.e., 1 ml of 1000X Amp or Carbenicillin;

or Kanamycin, 50 mg/L,  i.e., 1 ml of 1000 X Kan;

Antiform, one tiny drop (don't add more than 5 ul).

50 X 2G (g/L)

Glucose, 200;
Glycerol, 100;

Water to 1 L;

Filter sterilize.

(1X: glycerol, 2.0; glucose, 4 or 5)

20 X P (g/L)

K2HPO4, 10;

KH2PO4, 125;

(NH4)2HPO4, 40;

MgSO4, 20;

Water to 1L;

Autoclave.

(1 X: K2HPO4, 0.5; KH2PO4, 2.5; (NH4)2HPO4, 2.0; MgSO4, 1.0)

Trace metal solution (g/L):

FeCl3·6H2O, 0.162;

ZnCl2·4H2O, 0.0144;

CoCl2·6H2O, 0.12;

Na2MoO4·2H2O, 0.012;

CaCl2·2H2O, 0.006;

CuSO4·5H2O, 1.9;

H3BO3, 0.5;

HCl (37%, w/v), 37 ml/L

Filter sterilize

TB +GP

12 g tryptone

24 g yeast extract,

4 ml glycerol, water to 900 ml, autoclave

Then add 20 mL filter sterilized 1 M KH2PO4 and 80 mL filter sterilized 1 M K2HPO4

2XYT + GP

10 g yeast extract
16 g tryptone (or peptone)
5g NaCl
4 ml glycerol, water to 900 ml, autoclave

Then add 20 mL filter sterilized 1 M KH2PO4 and 80 mL filter sterilized 1 M K2HPO4

LB + GP

5g yeast extract
10 g tryptone (or peptone)
10 g NaCl
4 ml glycerol, water to 900 ml, autoclave

Then add 20 mL filter sterilized 1 M KH2PO4 and 80 mL filter sterilized 1 M K2HPO4

For Plasmid Production

1 Liter
K2HPO4, 12.54 g; 
KH2PO4, 2.31 g; 
D-Glucose, 4.0g; 
NH4Cl, 1.0g; 
Yeast extract, 12.0g;
NaCl, 5.0g; 
MgSO4, 0.24g;