Hot Start PCR QC

=

TREC ===

TREC_HDimer_F

5'-CACATCCCTTTCAACCATGCTG -3'

LENGTH 22

GC CONTENT 50 %

MEL TTEMP 56.6 ºC

TREC_HDimer_R

AGGGCCCGTGCCAGCTGCAG

LENGTH 20

GC CONTENT 75 %

MELT TEMP 68.2 ºC

Delta G: -3.55 kcal/mole Base Pairs3

5' CACATCCCTTTCAACCATGCTG
                     |||                 
3'                    GACGTCGACCGTGCCCGGGA

Actin

Actin_HDimer_F:

GGGCTTCTTGTCCTTTCCTTCCC

LENGTH 23

GC CONTENT 56.5 %

MELT TEMP 60 ºC

Actin_HDimer_R:

5'-GCTCATTGTAGAAGTACTTCAGGG -3'

LENGTH 24

GC CONTENT 45.8 %

MELT TEMP 55 ºC

Delta G: -6.14 kcal/mole Base Pairs3

5'GGGCTTCTTGTCCTTTCCTTCCC
                     |||                    
3'                    GGGACTTCATGAAGATGTTACTCG

500 bp

HDimer_F3

AGCTCGTGAAACACAAGCCC

LENGTH 20

GC CONTENT 55 %

MELT TEMP 58.2 ºC

Delta G: -6.14 kcal/mole Base Pairs3

5' AGCTCGTGAAACACAAGCCC
                   |||                 
3'                  GGGACTTCATGAAGATGTTACTC

tatagcatatgaatgctgcacagaatccttggaattcctgtcaacaaaggtgatgccacatccctttcaaccatgctgacacctctggtttttgtaaaggtgcccactcctgtgcacggtgatgcataggcacctgcaccccgtgcctaaaccctgcagctggcacgggccctgtctgctcttcattcaccgttctcacgagttgcaataagttcagccctccatgtcacactgtgttttccatcGGATCCTTGCTGTCATCTCTTGTGGGCTGTAATCATCGTCTAGGCTTAAGAGTAATATTGCAAAACCTGTCATGCCCACACAAATCTCTCCCTGGCATTGTTGTCTTTGCAGATGTCAGTGAAAGAGAACCAGCAGCTCcacttgttg agctcgtgaaacacaagcccaaggcaacaaaagagcaactgaaagctgttatggatgatttcgcagcttttgtagagaagtgctgcaaggctgccATGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGtaggggagctggctgggtggggcagccccgggagcgggcgggaggcaagggcgctttctctgcacaggagcctcccggtttccggggtgggggctgcgcccgtgGGTACCctcagggcttcttgtcctttccttcccagGGCGTGATGGTGGGCATGGGTCAGAAGGATTCCTATGTGGGCGACGAGGCCCAGAGCAAGAGAGGCATCCTCACCCTGAAGTACttctacaatgagc