Hot start reverse transcriptase
Jump to navigation
Jump to search
https://jast-journal.springeropen.com/articles/10.1186/s40543-015-0063-4
M-MLV RT Atapmer 5′-CUUACCACGCGCUCUUAACUGCUAGCGCCAUGGCCAAAACU-3′
A 3′ phosphorylation should be added to the oligo to eliminate any possible elongation of the aptamer.
at aptamer concentration of 25 μM allowed full RT activity at 55 °C but significantly reduced RT activity at 31 °C