Ligase Buffers

1X T4 DNA Ligase Reaction Buffer

50 mM Tris-HCl
10 mM MgCl2
1 mM ATP
10 mM Dithiothreitol
pH 7.5 @ 25°C

1X T7 DNA Ligase Reaction Buffer

66 mM Tris-HCl
10 mM MgCl2
1 mM ATP
1 mM DTT
7.5 % PEG 6000
pH 7.6 @ 25°C