MCF-10A

Mid-passage MCF-10A cells, human breast epithelial cells obtained from the Barbara Ann Karmanos Cancer Institute (passage 120), were cultured in DMEM:F12 media (1:1) supplemented with 15% equine serum, insulin (10 ug/ml), epidermal growth factor (20 ng/ml), cholera toxin (100 ng/ml), and hydrocortisone (0.5 ug/ml). Parental cells were maintained with penicillin (100 units/ ml), streptomycin (100 ug/ml), and fungizone (0.5 ug/ml). Cells were passaged at 1:3 to 1:6 every 3– 4 days.