MKpnI 1715 1868 1483 1900 1654 1901 1464 1902 1500 1903 1904

Protein Expression

2013/10/30
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	express
1	pUS40-mKpn1+pTQ-Kpn1-His	mKpnI	1861+1862	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT o/n	Ni beads		no
2	pT7A3-Pfu-sso7d	Pfu-sso7d	1715	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.47 / 98695.05	yes
3	pT7A30-fMMLV	fMMLV	1868	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.91 / 75516.97	yes
4	PageRuler Plus Prestained protein ladder	250, 130, 100, 70(red), 55, 35, 25(red), 15, 10 (yellow)
5	pT7A3-T4_DNA_Ligase	T4_DNA_Ligase	1483	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.14 / 55292.65	yes
6	pT7A3-6H-sso7d-T4-DNA-Ligase	T4_DNA_Ligase	1900	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	7.95 / 64443.13	yes
7	pT7A3-T3 DNA ligase	T3 DNA ligase-2	1654	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.14 / 39350.70
8	pT7A3-6H-sso7d-T3-DNA-Ligase-2	T3 DNA ligase-2	1901	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.16 / 52085.67	yes
9	pT7A3-T7 DNA ligase	T7 DNA ligase	1464	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	41 kDa , pI=5.27	yes
10	pT7A3-6H-sso7d-T7-DNA-Ligase	T7 DNA ligase	1902	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.09 / 50283.49	yes
11	pT7A3-T3-ligase	T3 DNA ligase	1500	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT o/n	Ni beads	5.14 / 39350.70	no
12	pT7A3-6H-sso7d-T3-DNA-Ligase	T3 DNA ligase	1903	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.10 / 49697.48	no
13	pT7A3-T3-DNA-Ligase-sso7d	T3 DNA ligase	1904	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.84 / 48436.09	no