MS 4 18 2017
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run SDS-PAGE gel 1567 EXO/1483 T4 DNA ligase 04/18/2017
| 1567 |
1483 | |||||||||||
| Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| sample |
23 |
24 |
26 |
27 |
28 |
MW |
16 |
18 |
19 |
24 |
28 |
32 |
| uL |
8 |
9 |
5 |
8 |
13 |
10 |
4 |
0.2 |
0.2 |
1 |
3 |
5 |
| ddH2O |
5 |
5 |
10 |
5 |
\ |
\ |
10 |
15 |
15 |
10 |
10 |
10 |
1567 2013/4/5 lane8 http://excellgen.net/eln/index.php/1696_1707_1567_1673_1697
Dialysis of sample 1567-24.
0.20mg/ml 4.8ml, total 0.96mg. Wash#1 0.14mg/ml, 1ml.
1315 RNA ligase elute with Buffer B 04/18/2017
| 0.19 |
0.16 |
0.21 |
0.28 |
0.50 |
(1~5) |
| 0.80 |
1.17 |
1.10 |
1.37 |
1.49 |
(6~10) |
| 0.60 |
0.87 |
1.11 |
1.44 |
1.85 |
(11~15) |
| 2.15 |
2.61 |
3.03 |
3.20 |
3.06 |
(16~20) |
| 3.09 |
3.11 |
3.00 |
2.64 |
2.49 |
(21~25) |
| 2.24 |
1.97 |
1.95 |
2.10 |
1.45 |
(26~30) |
| 1.66 |
1.19 |
1.24 |
1.12 |
1.07 |
(31~35) |
| 0.99 |
1.02 |
0.88 |
0.81 |
0.84 |
(36~40) |
| 1.18 |
0.86 |
0.78 |
0.72 |
0.68 |
(41~45) |
| 0.62 |
0.61 |
0.57 |
0.55 |
0.56 |
(46~50) |
| 0.57 |
(51~55) | ||||
| 0.45 |
(56~60) | ||||
| 0.29 |
(61~65) | ||||
| 0.24 |
(66~70) |
3~70


