MS 5 19 2017

Prepare competent cell (Nico21)

Run SDS-PAGE gel of 121-1715 Pfu and 122-1315 Mth RNA Ligase.

	1715 Pfu 98.6						1315 Mth RNA Ligase 45.3
Number	1	2	3	4	5	6	7	8	9	10	11	12
sample	14	15	17	18	19	MW	25	27	33	35	36	44
uL	0.4	1.0	0.9	0.4	0.8	10	0.9	1.0	0.5	0.2	0.2	0.9
ddH2O	15	15	15	15	15	\	15	15	15	15	15	15

1~5:1715

6:MW

7~12:1315

Conclusion: forward sample 14 and 15 of 1715, and 33~44 of 1315 to the next step.

Transfaction reagent 50ul/tube

QC of 1542 Native ToughTaq.

1	2	3	4	5	6	7
positive control	1mg/ml	0.1mg/ml	10ug/ml	1ug/ml	0.1ug/ml	negative control
0.5ul	0.5ul	0.5ul	0.5ul	0.5ul	0.5ul	0

20ul/tube:

dNTP	template	F	R	10x Buffer	H2O	Taq
1ul	0.1ul	1ul	1ul	2ul	14.4ul	0.5ul

Conclusion: need to do again with less cycle.