MS 6 7 2017
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Culture 1498 Taq DNA Ligase 200ml.
Culture 1497 sso7d-ToughTaq 8L, induce with IPTG.
Spin down 1946 TdT bovine.
Prepare LB media 20L.
Prepare LB/K agar and LB/K/C agar.
Plasmid transformation. 1942 phi 29, 1601 6His-GST-i3C, 1518 T3 Ligase, 1765 T4 DNA Polymerase.
Wash Ni column (load with 1464 T7 DNA Ligase) with Ni washing buffer / Tris-HCl / Buffer B, dilute and load onto QHP column.
Elute 1464 T7 DNA Ligase with Buffer B 06/07/2017
| -0.19 |
-0.21 |
-0.02 |
1.36 |
2.34 |
(1~5) |
| 7.36 |
3.66 |
1.11 |
0.65 |
0.34 |
(6~10) |
| 0.15 |
0.09 |
0.06 |
0.03 |
0.01 |
(11~15) |
Collect 4~11.
1708 Cre Recombinase 1:1 dilute with SP Dilution Buffer. SP FF column wash with SP Elution Buffer and SP Wash Buffer, then load Cre onto SP FF column.