MS 6 9 2017
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1518 T3 Ligase, 1601 6His-GST-i3C, 1765 T4 DNA Polymerase, 1942 phi 29 pick up single colony to culture in LB/K/C media for small scale screening.
Spin down 1518 T3 Ligase, 1601 6His-GST-i3C, 1765 T4 DNA Polymerase, 1942 phi 29 to make glycerol stock. Store at -80oC.
Spin down 1498 Taq DNA Ligase, store at -20oC.
Run SDS-PAGE gel of 1464 1464 T7 DNA Ligase 06/09
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
|
sample |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
MW |
|
uL |
9.1 |
3.1 |
0.8 |
0.4 |
0.3 |
0.9 |
1.5 |
3.7 |
10 |
|
ddH2O |
5 |
10 |
15 |
15 |
15 |
15 |
15 |
10 |
\ |
Collect 4~8 forward to the next step. Dialysis.
23.2mg/ml, 4.5ml, 104.4mg. Purity>95%.
Wash Ni column (load with 1946 TdT bovine) with Ni washing buffer / Tris-HCl / Buffer B.
Elute 1946 TdT bovine with Buffer B 06/09/2017
| -0.05 |
-0.21 |
-0.07 |
0.81 |
2.81 |
(1~5) |
| 2.05 |
1.09 |
0.71 |
0.50 |
0.33 |
(6~10) |
| 0.23 |
0.18 |
0.13 |
0.05 |
0.01 |
(11~15) |
collect 4~12