MS 7 12 2017
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Wash Ni column (load with 818 pHis-GST-His-Flag-His) with Ni washing Buffer/ Tris-HCl/ Buffer B
Elute 818 pHis-GST-His-Flag-His with Buffer B 07/12/2017
| -0.30 |
-0.26 |
0.05 |
1.95 |
2.26 |
(6~10) |
| 1.68 |
1.18 |
0.85 |
0.62 |
0.43 |
(11~15) |
| 0.30 |
0.20 |
0.14 |
0.08 |
0.05 |
(16~20) |
collect 9~16.
Load 1782 Tth-Ligase onto QHP column.
Elute 423 DeepVent-sso7d from QHP column.
Run SDS-PAGE gel of 423 DeepVent-sso7d 8.69 / 99.5 07/12/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
|
sample |
14 |
15 |
16 |
17 |
18 |
MW |
20 |
22 |
|
uL |
15 |
4.6 |
2.7 |
2.7 |
4.0 |
10 |
5.0 |
5.9 |
|
ddH2O |
\ |
10 |
10 |
10 |
10 |
\ |
10 |
10 |
Forward lane 1~2 to the next step.
Dialysis of 1567 T5-Exo. 1.5mg/ml, 8.0ml.