MS 7 13 2017
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Load 818 pHis-GST-His-Flag-His onto QHP column.
Elute 1782 Tth-Ligase from QHP column.
Run SDS-PAGE gel of 1782 Tth-Ligase 6.56 / 79.1 07/13/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
|
sample |
MW |
FT1 |
14 |
17 |
18 |
23 |
FT2 |
|
uL |
10 |
15 |
10 |
0.5 |
0.22 |
0.9 |
15 |
|
ddH2O |
\ |
\ |
5 |
15 |
15 |
15 |
\ |
FT1: flow through after Ni column.
FT2: flow through after QHP column.
Forward lane 5~6 to the next step.
Dialysis of 423 DeepVent-sso7d. 1.50mg/ml, 8.0ml.