MS 7 6 2017
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Culture 1782 Tth Ligase 8L, induced with IPTG.
Homogenize 423 pET30b-DeepVent-sso7d, load onto Ni column.
Load 2462 Tn5 transposase onto QHP column.
Wash Ni column (load with 1567 Exo) with Ni washing buffer/ Tris-HCl/ Buffer B.
Elute 1567 Exo with Buffer B 07/06/2017
| -0.25 |
-0.26 |
-0.25 |
-0.25 |
-0.23 |
(1~5) |
| -0.10 |
1.82 |
1.04 |
0.36 |
0.15 |
(6~10) |
| 0.07 |
0.04 |
0.03 |
0.01 |
0.01 |
(11~15) |
collect 7~11.
Elute 1811 vPol
Run SDS-PAGE gel of 1811 vPol 6.63 / 69.2 07/06/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
sample |
8 |
10 |
12 |
14 |
15 |
16 |
17 |
MW |
18 |
19 |
20 |
21 |
|
uL |
5.9 |
3.9 |
4.0 |
2.1 |
0.23 |
0.38 |
0.3 |
10 |
0.44 |
0.55 |
0.7 |
0.1 |
|
ddH2O |
10 |
10 |
10 |
15 |
15 |
15 |
15 |
\ |
15 |
15 |
15 |
15 |
forward lane 1~7 to the next step.
Dialysis of 1995 Kfu. 4.18mg/ml, 4.5ml, purity > 90%