MS 7 7 2017
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Wash Ni column (load with 423 DeepVent-sso7d) with Ni washing buffer/ Tris-HCl/ Buffer B.
Elute 423 DeepVent-sso7d with Buffer B 07/07/2017
| -0.29 |
-0.26 |
-0.14 |
0.99 |
1.27 |
(6~10) |
| 0.94 |
0.80 |
0.70 |
0.64 |
0.61 |
(11~15) |
| 0.57 |
0.50 |
0.49 |
0.44 |
0.40 |
(16~20) |
| 0.39 |
0.37 |
0.30 |
0.28 |
0.24 |
(21~25) |
| 0.22 |
0.22 |
0.16 |
0.11 |
0.08 |
(26~30) |
collect 9~27.
Elute 2462 Tn5 transposase
Run SDS-PAGE gel of 2462 Tn5 transposase 8.41 / 83.8 07/07/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
|
sample |
22 |
23 |
24 |
25 |
26 |
MW |
27 |
28 |
29 |
30 |
|
uL |
6.3 |
8.4 |
7.2 |
5.6 |
5.3 |
10 |
6.3 |
7.2 |
8.4 |
10 |
|
ddH2O |
10 |
5 |
5 |
10 |
10 |
\ |
10 |
5 |
5 |
5 |
forward lane 1~4 to the next step.
Dialysis of 1811 vPol. 36.0mg/ml, 10.0ml, purity > 90%