MiniPrep Column Regeneration

1. Save the collection tubes and columns after elution of DNA
2. Fill the column with 700 ÎŒl of 1 M HCl
3. Cap and store in an airtight container for at least 24 hours (less than a month)
4. Wash the columns and collection tubes in a large beaker of water
5. Assemble the column and collection tube and wash the column with 700 ÎŒl of DI water, discarding the water
6. Repeat
7. Fill the column with 700 ÎŒl of buffer QBT and spin down, discarding the buffer
8. Place the columns in an airtight plastic bag for storage
9. Wash the collection tubes, air dry, and store them for reuse