Ni Buffer B

25 mM Tris-HCl, pH8.0, 100 mM NaCl,  150 mM imidazole, 1 mM b-ME

11.7 g solid NaCl

or 50 ml of 4 M NaCl

6.4 g Trizma HCl + 1.2g Trizma Base

or 50 ml of 1M Tris Buffer, pH 8.0

140 ul beta-ME

20 g imidazole

Add 1 L water to dissolve.

Alternatively, add 100 ml glycerol (final conc is 5%)

Add  ddH20 to make 2 L