Oligo Stock
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1. after receiving oligo, check amount in nmole.
2. add 100 ul TE or EB to 10 nmole oligo (e.g., add 140 ul TE/EB to 14 nmole). Votex and let it sit at RT for 10 min. If it is on plate, do not mix. This is 100 uM stock.
3. Take 10 ul to a new tube with 90 ul H2O, this is 10 uM stock.
4. Store 100 uM stock in -20 oC freeze.
5. Store 10 uM stock in your box. Use 10 uM stock for PCR.