PBS Powder
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1 L of PBS
[edit]To make 1 L of PBS, you will need the following amounts of each ingredient:
Small amounts of dilute HCl and/or NaOH to pH the final solution
800 mL of distilled water
8 g of NaCl (sodium chloride)
0.2 g of KCl (potassium chloride)
1.44 g of Na2HPO4 (disodium phosphate)
0.24 g of KH2PO4 (monopotassium phosphate)
The final concentrations of each compound should be 137 mm NaCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, and a pH 7.4
10 L
[edit]80 g of NaCl (sodium chloride)
2 g of KCl (potassium chloride)
14.4 g of Na2HPO4 (disodium phosphate)
2.4 g of KH2PO4 (monopotassium phosphate)