PCR Enhancer Mix

1, 5X Enhancer:

4 M betaine, 10 mM DTT, and 10% DMSO, 55 ug/ml BSA 

15 ml: 7 g betaine, 150 ul 1 M DTT, 1.5 ml DMSO, 83 ul of 10mg/ml BSA

stable at 20 C for at least 3 months

Taq reaction buffer containing final concentrations of 65 mM TrisHCl, 16.6 mM (NH4)2SO4, 3.1 mM MgCl2, and 0.01% (v/v) Tween 20 at a pH of 8.8 

2, Real-time PCR enhancer

PCR mix supplemented with 1 M 1,2-propanediol and 0.2 M trehalose (PT enhancer).