PCR Stabilizer

https://www.google.com/patents/US5861251

Performance of PCR is not affected by the addition of stabilizers such as ammonium sulfate, glycerol, glucitol, PEG-8000 or Thesit, provided that both ammonium sulfate and glycerol are not added to the mixture simultaneously.

lyophilization does not have an effect on PCR, provided that ammonium sulfate is not added to the PCR mixture

the addition of stabilizer (such as 4% glycerol, 20 mM glucitol, 0.1% PEG-8000 and 0.1% Thesit)  improves heat stability of PCR; and, the lyophilized PCR mixture containing 20 mM glucitol provides a highly improved heat stability.

the addition of stabilizer (100 mM glucitol) and lyophilization resulted in heat stability of a PCR mixture Glucitol which is a polyol, conferred the most improved heat stability on the lyophilized PCR mixture.

the lyophilized reaction mixtures containing 20 mM of glucose, mannitol, galacitol or glucitol as a stabilizer,  improved heat stability of the lyophilized PCR mixture

bromophenol blue, xylene cyanole, bromocresol red, and cresol red ca be added to  in the preparation of the lyophilized PCR mixtures., but not methyl green,