PKA ELISA 4-8-2011

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Notebook Zhen Tao #11

Page 2101~2102

all samples are diluted 1/400 with blocking solution;
Control: only 2nd antibody (anti-human) was used;
sample 29 and 447667~447669: prostate cancer people serum;
sample 447670~447672: colon cancer people serum;
sample 447673~447675: breast cancer people serum;

sample 31~146 are normal people serum;
positive: anti-PKA-C-alpha is used

15 min, instead of 30 min was used here after adding TMB solution.

samples
 OD450-1
 OD450-2
 OD450-3
 average of all normal serum
 average of cancer sample
 ration of cancer/normal
control
 0.005
 0.006
 0.005


447667
 0.856
 0.841
 0.850
0.844
 3.706
447668
 0.501
 0.508
 0.526
0.506
 2.224
447669
 0.323
 0.331
 0.309
0.316
 1.387
447670
 0.290
 0.289
 0.290
0.284
 1.249
447671
 0.143
 0.146
 0.152
0.142
 0.622
447672
 0.468
 0.505
 0.547
0.501
 2.202
447673
 0.895
 0.545
 0.585
0.670
 2.942
447674
 0.153
 0.160
 0.169
0.155
 0.682
31
 0.190
 0.193
 0.201
 0.228

32
 0.279
 0.281
 0.318

33
 0.232
 0.255
 0.242

34
 0.177
 0.168
 0.151

35
 0.412
 0.393
 0.418

39
 0.205
 0.208
 0.211

40
 0.106
 0.124
 0.124

132
 0.285
 0.286
 0.260

133
 0.167
 0.172
 0.182

135
 0.151
 0.153
 0.166

136
 0.058
 0.590
 0.156

146
 0.313
 0.291
 0.269

positive
 1.718
 1.824
 1.869




--Ztao 13:03, 8 April 2011 (EST)


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