PLVGP

Cloning Method

to PCR out GreenPuro
Xho1 22nt IRES
atcg CTCGAG TTTATAATTTCTTCTTCCAGAA GCCACCatggagagcgacg

GFP-Puro-F
atcgCTCGAGTTTATAATTTCTTCTTCCAGAAGCCACCatggagagcgacg
GFP-Puro-R Mlu1
cccgcaagcccggtgcctga ACGCGT atcg
oligo

cgatACGCGTtcaggcaccgggcttgcggg

PCR out using pGreenPuro as template, digest with Xho1+ Mlu1, ligate with Xho1+ Mlu1 digested pLVX-IRES-mCherry-Plus2

Lane 5: Clone #1 digest with Xho1+ Mlu1

Lane 6: Clone #2 digest with Xho1+ Mlu1

Lane 7: Clone #3 digest with Xho1+ Mlu1

Lane 8: Clone #4 digest with Xho1+ Mlu1