PLenti-mKate2
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SubCloning of mkate2 to pLentiGP
[edit]Purpose: Subcloning of mkate2 to pLentiGP from pet28b-mkate2.
Date: 2011-6-20 to 2011-6-23
Notebook: David, Robert
Page: 001543
Clone ID: 220
Experimenters: David, Robert, Haiyan http://192.168.1.11/eln/index.php?title=PLentiGP-PKA-Alpha&action=edit
Results
[edit]Subcloning
[edit]Date: 2011-6-23
Digest pET28b-mKate2 with BamH1 + Xho1
Run gel and cut out insert
Digest pLentiPlus with BamH1 + Xho1
Run gel and cut out vector combine and purify using spin column
Ligate and transform STBL3
Colony Screening
[edit]Date: 2011-6-23
Make miniprep and digest pLenti-mKate2 with BamHI + Xho1
Lanes 1 - 11: pLenti-mKate2, BamHI + Xho1
Result intepretation/Summary
[edit]- Lane 4 has the correct number of bands.
Conclusion
[edit]Restriction digestion shows that clone #4 is good.
[edit]
David.Hu 11:33, 23 June 2011 (EST)