Proteases

6His-3C	0	1797	1510	pCold-6His-3C-MBP	6His tagged wildtype native 3C fused to MBP, MBP at C-terminus	N-6His, C-MBP	PCR	wt	6.01 / 62.6	6XHis-3C with small amount truncted 6XHis-3C-MBP fusion
6His-GST-3C	0	1613	1465	pET28-cold-GST-3C	native 3C fused to 6X His-GST	N-His-GST	PCR	wt	6.99/47.4	full length
6His-3C	0	1729	1697	pCold-TF-(E3C)-3C	TF fused to autocleavable 3C protease	N-His-TF	sub	wt	8.47 / 21.4	full length, but TF has His tag too
6His-3C	0	1697	1240	pCold-GST-(3c)-wt3C	autocleavage wild type 3C protease, fused to GST tag	N-His, GST at N, but autocleable	PCR	wt	8.48/21.4	full length
6His-3C	0	1606	1510	pET28b-Cold-3Cwt	wild-type 3C	N-6His	Sub	wt	8.76 / 21.6	-
6XHis-GST-3C	0	1601	1554	pT7A3-6His-GST-i3C	improved native HRV 3C	N-6XHis-GST, C-XHis	sub	my algorithm	6.41 / 50.8	high
6His-MBP-3C	0	1554	pT7A3-MBP-i3C	improved native HRV 3C	N-6XHis-MBP, C-6XHis	GS	my algorithm	5.86 / 65.4	No, only MBP expressed
6His-MBP-3C	0	1511	1197	pT7A3-MBP.Synthetic-3C	E coli version of 3C	N-6XHis-MBP	sub	Invitrogen	5.81 / 63.9	no
6His-MBP-3C	0	1510	1240	pT7A3-MBP.Native-3C	wt 3C fused to 6XHis-MBP	N-6XHis-MBP	sub	wt	5.77 / 63.6	no
6His-MBP-3C	0	1420	pDB.His.MBP.3C	wt 3C, N-6His_MBP	N-6XHis-MBP	GS	wt	5.93 / 64.2	-
6His-3C	0	1240	pET28b-3C	invitrogen E coli	N-6XHis	GS	Invitrogen	8.76 / 22.6	-
6His-GST-3C	0	1197	pDB.His.GST-3C	invitrogen E coli	N-6His-GST	GS	Invitrogen	6.39 / 49.0	-
MBP-3C-6His	0	2092	1601	pT7A3-MBP-i3C-6H	improved wt 3C fused to N-MBP and C-6His, no N-his tag	N-MBP, C-6His	PCR	my algorithm	5.63 / 64.9	need to scren