Purification Tags
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ceramic fluorapatite-binding peptides: KPRSVSG
PA tag ( from human podoplanin PLAG domain)
GVAMPGAEDDVV
MPA tag
GDGMVPPGIEDK
Fc-binding hexamer peptides (HWRGWV) --binding human IgG
FYWHCLDE
FYCHTIDE
FYWHCLDE-Sepharose for human IgG purification
Ligand binding competition revealed that the binding sites on hIgG for the three octapeptides were similar to those for Protein A. Adsorption isotherms revealed that hIgG binding capacity was in the range of 64-104mg/mL drained gel in the order of FYWHCLDE>FYCHWALE>FYCHTIDE