Q5 DNA Polymerase

10X Buffer

Potassium chloride, 2.5-5.0%
Proprietary Component, 4-6.5%
Ammonium Sulfate, 1-2.5%
Tris 1.2%
Proprietary Component 1-2.5%

QC Assay condition (not PCR)

250 mM TAPS-HCl (pH 9.3 @ 25°C), 500 mM KCl, 20 mM MgCl2, 10 mM β-mercaptoethanol

High GC enhancer

Glycerol 25%
DMSO 20%
Proprietary Component, 2-5%

Enzyme has Nucleic acid-based aptamers:

5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502

Fusion:  6,627,424, 7,541,170, 7,670,808, 7,666,645

PCR condition:

200 μM dNTPs

1.5 mM Mg