QPCR

10x Platinum taq buffer (Invitrogen)  2.5μl 
50mM MgCl 2 (→ 1.5mM)  0.75μl
template DNA  2.5μl
10μM DLP (→ 250nM)  0.625μl
50x Rox (Invitrogen; → 1x)  0.5μl
c_qPCR_v2.1 (→ 300nM)  0.75μl
PE_qPCR_v2.2 (→ 300nM)  0.75μl
2.5mM dNTPs (→ 200μM)  2μl
Platinum Taq (Invitrogen; → 0.04 units / μl)  0.1μl
H2O  14.525μl

94C for 2 min
94C 15sec
62C 15sec
72C 32sec
x 40