Rapid Run Agarose Gel Buffer

20x RapidRun Agarose Buffer

• 1.78 M Tris

• 0.57 M Taurine

• 0.01M EDTA

adjust pH to 8.9, filter.

Reagent Supplier purity Cat. no. approx. price
Taurine (125.15 g/mol) Sigma ≥99%: T0625-500G USD162.00
Tris base (121.14 g/mol) Sigma ≥99%: T1378-5KG USD291.00
EDTA (292.24 g/mol) SysBio kitchen ≥99%: NA NA

For 5 L of 20x buffer combine:
1113.8 g of Tris
345.25 g of Taurine
14.6 g (or 50 mL of 1M EDTA)

bring to 4.5 L with H2O. Adjust pH to 8.9 and filter.