Restriction Digestion

Digestion

Use 5 ul miniprep for  digestion

1. make mix, per reaction

1.5 ul FastDigestion Buffer

0.3 ul enzyme 1

0.3 ul enzyme 2 (if required)

5 ul miniprep

H2O to 15 ul

2. Incubate at 37 oC  for 30 min in an incubator 

3. Add 5 ul 6X GRGreen loading buffer

4. Run on 1% agarose gel

5. Take a picture using blue light transilluminator 

6. Process gel picture and upload to ELN

7. Document all experimental details