RunBlue Buffer
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20x RunBlue SDS Running Buffer
[edit]
0.8 M Tricine
1.2 M Tris
2% SDS
50 mM Sodium Bisulfate
71.7g Tricine (free base)
72.6g Tris (free base)
10.0g SDS
2.5g Sodium Bisulfate
500mL dd water
pH 8.2-8.3 at 25C.
Notes: Add everything but the SDS and fill to ~450mL, make 800mL.
1X RunBlue SDS Running Buffer
[edit]20x RunBlue SDS Running Buffer: 40mL
800x Anti-oxidant (NXA30010): 1 mL
ddH2O: 759 mL
20x RunBlue SDS Fast Running Buffer
[edit]0.6 M MOPS
1.2 M Tris
2% SDS
50 mM Sodium Bisulfate – optional (this makes it reducing)
| MOPS (free acid) | 62.8g | |
| Tris (free base) | 72.6g | |
| SDS | 10.0g | |
| Sodium Bisulfate | 2.5g | or 6.5 g |
| dd H2O | 500mL |
pH 8.2-8.3 at 25C.