SD Polymerase

Although Bst LF works at higher temperatures, it is not truly thermostable at temperatures that may be required for DNA denaturation and “hot-start” LAMP. There have been reports of two thermostable polymerases that could potentially be included in a denaturation step in IA reactions such as LAMP: OmniAmp, a viral polymerase from PyroPhage 3173 with DNA polymerase and reverse transcriptase activities,(29) and SD Polymerase, a mutant of the well-known Thermus aquaticus (Taq) polymerase.(30) However, neither of these polymerases has been validated for hot-start LAMP, because of either the lack of sufficient thermostability to survive denaturation steps (OmniAmp) or insufficient strand displacement activity for consistent LAMP performance (SD Polymerase).

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