Split Intein

Split Inteins Alignment

Wt
CVYGDTMVETED GKIKIEDLYKRLA
MFRTNTNNIKI LSPN GFSNFNGIQKVERNLYQHIIF DDDTEIKTSINHPFGKDKI LARDVKVGDY LNSK KVLYNELVNENI FLYDPINVEKESLYITNGVVSHNC

N46D, L55Q
CVYGDTMVETED GKIKIEDLYKRLA

MFRTNTNNIKI LSPN GFSNFdGIQKVERNqYQHIIF DDDTEIKTSINHPFGKDKI LARDVKVGDY LNSK KVLYNELVNENI FLYDPINVEKESLYITNGVVSHNC

Int-N
HindIII Not1 Xho1 Sal1
AGCTC C AAGCTT GCGGCCGCA ctcgag TGTGTATATG GTGATACAAT GGTGGAAACT GAAGATGGGA AAATTAAAAT CGAGGATCTG TACAAACGTC TGGCAtaa GT CGA C atacac

Int-C

ATGTT TCGCACGAAC ACCAATAATA TCAAAATTTT GAGCCCGAAC GGTTTTTCAA ACTTTGATGG TATCCAGAAA GTCGAGCGCA ACCAGTATCA GCATATTATC TTCGATGATG ATACTGAAAT TAAAACGAGC ATTAACCATC CTTTCGGGAA AGATAAGATT CTGGCACGGG ATGTCAAAGT AGGTGATTAT CTGAATAGTA AAAAAGTCCT GTACAATGAA CTGGTTAACG AGAACATCTT TCTGTATGAT CCGATTAACG TCGAAAAGGA AAGCCTCTAT ATCACCAACG GGGTTGTGTC GCATAACTGC

Y3S, N46D, L55Q
CVsGDTMVETED GKIKIEDLYKRLA
MFRTNTNNIKI LSPN GFSNFdGIQKVERNqYQHIIF DDDTEIKTSINHPFGKDKI LARDVKVGDY LNSK KVLYNELVNENI FLYDPINVEKESLYITNGVVSHNC