Subramanian 1 shRNA-3

Notebook: Zhen Tao

Page: 001931

Transfection reagent: PEIMAX, 32.4 ul/flask

Packaging Mix: V:G = 1:3 (prepared by mixing 0.1 ug/ul corresponding DNA). 108 ul/flask

Plasmid (PNPLA2_367881 or PNPLA2_367882): 7.2 ug/flask.

Use 1 ml DMEM(-) to prepare transfection reagent or plasmid, then add transfection reagent to plasmid and keep at room temperature for 20 min; add it to flask drop by drop.

one day after transfection --Ztao 14:41, 11 February 2011 (EST)

6 day after transfection --Ztao 09:21, 16 February 2011 (EST)