Sybr Green Mix
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10 X PCR Buffer: 120 mM Tris-HCl, pH 8.3, 500 mM KCl, 50 mM MgCl2, 1.5 M Trehalose, 1M Betaine, 2 mg/ml BSA, 2% v/v Tween-20
SYBR-G 1:1000 2.5 µl 1U
Hotstar Taq 0.5 µl 2,5 U
Rox (10 µM) 0.33 µl 0,1333 µM
MgCl2 (25mM) 1 µl 1mM
dNTPs 10mM 0.5 µl 200µM
Triton X-100 0.375 µl 1.50%
DMSO 1.25 µl 5%