Synthesis PHP-DTBP-DSP Comparison

Comparison of DTBP vs DSP and PEI800 vs PEI18

0.4 mg/ul PEI10 in DMSO

6/22/14

Sample #	PHP suspension	PEI800-DTBP	PEI800-DSP	PEI1800	DSP 100mg/ml	DTBP 100mg/ml	Total
1	50			2.5	5
2	50			5	7.5
3	50			7.5	10
4	50		5		5
5	50		10		7.5
6	50		15		10
7	50	7.5*			5
8	50	10			7.5
9	50	15			10
10	50			2.5		5
11	50			5		7.5
12	50			7.5		10
13	50		5			5
14	50		10			7.5
15	50		15			10
16	50	7.5*				5
17	50	10				7.5
18	50	15				10

• add 7.5 ul because 5 ul is not enough to form soluble PHP-PEI800 complex

React RT o/n

6/23/14

Dilute 1 ul in 30 ul  10 mM Hepes, pH 7.0

use 3 ul to transfect 1 ug DNA, 293T cells.

6/24/14, take pictures