Synthesis PHP-S-S-PEI18
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Conjugate PHP tp PEI18 without DSP
[edit]| # | PHP | 0.4g ml/mlPEI | DBP | Cystamine | Observation |
| 1 | 50ul | 5 ul | 0 | 0 | smallest pellet after isopropanol ppt |
| 2 | 50 | 5 | 1 | 0 | |
| 3 | 50 | 5 | 2 | 0 | |
| 4 | 50 | 5 | 3 | 0 | |
| 5 | 50 | 5 | 1 | 5 ul | large white pellet after isopropanol ppt |
| 6 | 50 | 5 | 2 | 5 | largest white pellet after isopropanol ppt |
| 7 | 50 | 5 | 3 | 5 | pellet smaller than #5 |
react o/n at 50 oC.purify by isopropanol ppt. dissolve in DMSO.
7/16/14: make 1:10, 1:30 dilution using pH7.0 HEPES buffer, 10 mM for transfection, 1 ug DNA + 3 ul. 293 T
7/18/14: picture after 2 days.
7/19/14
7/20/14
7/21/14
Conclusion
[edit]Without DSP conjugation the transfection efficiency is low