Synthesis chlo-9Arg
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1. Disolve oligoarginine (15.4 mg, 10.8 umol) in 5 ml of anhydrous dimethylformamide.
2. Add 15.08 ul of TEA to the oligoarginine solution.
3. Add cholesterol chloroformate (24.3 mg, 54 umol) in 5 ml of anhydrous tetrahydrofuran slowly to the solution.
4. Stir the solution overnight.
5. Remove solvents by purging dry nitrogen gas through a glass transfer pipette.
6. Rinse off the remaining cholesterol chloroformate and TEA three times with 15 ml of diethyl ether each time.
7. Dialyse for 2 days using MWCO 500 (Spectrum) against deionized distilled water to remove free cholesterol.
8. 14 mg of Chol-R9 can be obtained after freeze drying.
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