Synthesis of PHP-s-s-TREN

Synthesis of PHP-s-s-TREN

DAP + DBP Mix: 75 mg DAP+ 150 ul MeOH, warm to 50 oC to dissolve, add 366 mg DBP-->350 ul

TREN: 180 mg TREN + 450 ul MeOH-->580 ul

Cystamine: 28 mg + 56 ul water-->73 ul, 1 mg/2.6 ul

	DBP	DAP	TREN	Cystamine
C1	61 mg	12.5 mg	30 mg	0
C2	61	12.5	30	1 mg, 2.6 ul
C3	61	12.5	30	2.5 mg, 6.6 ul
C4	61	12.5	30	5 mg, 13.4 ul
C5	61	12.5	30	7.5 mg, 20 ul
C6	61	12.5	30	10 mg, 26.8 ul

50 oC react o/n

Purify with 1 ml isopropanol, 2X, dissolve 500 ul H2O. Dilute with 10 mM HEPES, pH7.0, 1:10, 1:30, 1:50, 1:100

8/25/14

Transfection 293T cells, 1ug DNA /3 ul diluted TR

8/26/14. GFP too weak to see

8/27/14

8/28/14

8/29/14