TALEN Construction

https://boglab.plp.iastate.edu/node/add/single-tale

https://tale-nt.cac.cornell.edu/node/add/talen

http://talen-hit.cellectis-bioresearch.com/target/talenHitSearch

http://idtale.kaust.edu.sa/

http://taleffectors.com/

http://baolab.bme.gatech.edu/Research/BioinformaticTools/assembleTALSequences.html

Destination Vectors Information:

Assembly Method	Plasmid Name	Description	Addgene
Cermak et al.	pTAL1	TALE with natural bacterial activator domain	31031
	pTAL2	TALE with natural bacterial activator domain and no stop codon	31033
	pTAL3	TALEN with HIS3 marker	31034
	pTAL4	TALEN with LEU2 marker	31035
	Sangamo	Mammalian expression TALEN with 3x FLAG tag and (-152, +63) backbone	Coming Soon
	pTALE64G	Mammalian expression TALE with 3x FLAG tag, (-152, +63) backbone, VP64 activation domain, T2A sequence, and eGFP	Coming Soon
	pTALE64C	Mammalian expression TALE with 3x FLAG tag, (-152, +63) backbone, VP64 activation domain, T2A sequence, and AmCyan	Coming Soon
	pCS2TAL3-DD	3xFLAG tag, TALEN with obligate heterodimer DD FokI domain	37275
	pCS2TALE3-RR	TALEN with obligate heterodimer RR FokI domain	37276
	pTAL5-BB	Yeast expression TAL repressor with PGAL1 Promoter	36033
	pTAL6-BB	Yeast expression TAL repressor with PTEF1 Promoter	36034
	pCAG-T7-TALEN(Sangamo)-Destination	Mammalian expression TALEN with Sangamo backbone	37184
Reyon et al.	JDS70, 71, 74, 78	Mammalian expression TALENs with 3x FLAG tag and Sangamo backbone with different C-terminal RVDs	TALENkit
Briggs et al.	TALEN	Mammalian expression TALEN	Unknown