Taq-muts 636 637 638 639 640 641 643 644 645 649

2013-9-25

Lane	Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	Taq-muts				elute from Amylose column
2	Taq-muts				flow through from Amylose column
3	PageRuler Plus protein ladder
4	pT7A3-Xba1	636	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.94/28.4kd
5	pT7A3-Nco1	637	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.47/36kd
6	pT7A3-BglII	638	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.66/31kd
7	pT7A3-BamHI	639	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.47/29.6kd
8	pT7A3-EcoR1	640	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	5.96/36.5kd
9	pT7A3-EcoRV	641	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	8.62/32.7kd
10	pT7A3-Nhe1	643	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	6.06/42.7kd
11	pT7A3-SalI	644	5 ml, Nico21(DE3)plysH	0.2mM IPTG RT o/n	Ni beads	6.01/31kd
12	pT7A3-PstI	645	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	7.32/41kd
13	pT7A3-Xho1	649	5 ml, Nico21(DE3)plysH	0.2 mM IPTG RT o/n	Ni beads	8.66/32.3kd