Thermostable Polymerases

Polymerase Activity

Incorporation

Extension

Extension/Incorporation

Processitivity

Buffer

Half-life

Optimal Temp

Km(ug/ml)

5'-->3'

3-->5'

Mutation Frequency

Mutation Frequency

ATCC Number

Temp

Source

Price

Patent

Patent Date

Thermococcus sp. KS-1

Tks

9.38

11.46

1.22

1.04

0.00%

0.00%

55659

85.0�C

Guaymas Basin at a depth of 2000 m, Gulf of California, Mexico

354

5714373

Feb 3 1998

Thermococcus sp NA-1 DQ223723

60nt/s

20 mM Tris-HCl, pH 8.5, 1mM MgCl2, 60 mM KCl, 30 mM (NH4)2SO4, 0.1% Triton X-100.

3.5h at 100 oC, 12.5 h at 95 oC

-

+

2.2X10-4

Thermococcus celer

Tce

10.83

13.28

1.23

0.91

0.02%

0.00%

35543

85.0�C

Solfataric marine water hole on beach, Vulcano Island, Italy

354

Thermococcus siculi

Tsi

9.67

21.93

2.27

1.33

BAA-270

80.0�C

deep-sea hydrothermal vent, Okinawa Trough

354

Thermococcus kodakarensis D29671

KOD

6.8

6.69

0.98

270nt/s

2.65

0.02%

0.06%

BAA-918

60-100�C

KOD Plus

0.08%

Thermococcus litoralis

Vent

5210036

May 11 1993

Thermococcus zilligii

Thermococcus pacificus

Thermococcus gorgonarius

85

Thermococcus profundus

51592

80

Thermococcus thioreducens

BAA-394

85

sediments of Black Smoker, April 2001, Elena Pikuta

Thermococcus barossii

80

Pyrococcus furiosus D12983

Pfu

Pyrococcus horikoshii

700860D-5

290

Pyrococcus woesei

Deep Vent

68723

190

5352778

10/04/94

Pyrococcus woesei

49860

354

Pyrococcus sp GB-D U00707

as