Thermostable Reverse Transcriptase

1X Reaction Buffer
20 mM Tris-HCl pH 8.8 at 25°C

10 mM (NH4)2SO4

10 mM KCl

2 mM MgSO4

0.1% Triton X-100

200 uM dNTP

10X

200 mM Tris-HCl pH 8.8 at 25°C 

100 mM (NH4)2SO4 

100 mM KCl 

20 mM MgSO4 

1% Triton X-100 

ThermoFisher 5X Buffer for M-MLV, SuperScript®, SuperScript® II, and SuperScript® III reverse transcriptases

250 mM Tris-HCl, pH 8.3
375 mM KCl
15 mM MgCl2
0.1 M DTT