Transfection reagents comparison 12

Make new transfection reagent TR-11-2.

1 ug DNA, 3 ul TR, 50 ul dilution, 293 cells on 12 wells plate

TR11-2 1:10	1:20	1:30	1:40
TR3 1:10	1:20	1:30	1:40
TR11 1:10	1:20	1:30	Old TR3, 1:40

Picture taken on 4-28-11

Cell statuson 4-28-11

TR11-2 1:10 medium is clear	1:20  medium is clear	1:30 medium is turbid, cell lysed?	1:40 medium is turbid, cell lysed?
TR3 1:10 medium is turbid, cell lysed?	1:20 medium is turbid, cell lysed?	1:30 medium is turbid, cell lysed?	1:40 medium is clear
TR11 1:10 medium is turbid, cell lysed?	1:20 medium is turbid, cell lysed?	1:30 medium is turbid, cell lysed?	old TR3, 1:40 medium is clear

--Crm 09:14, 28 April 2011 (EST)