Transfection reagents comparison 2
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Notebook: Zhen Tao #100
Page: 001913
1 day after transfection
--Paul.xu 14:14, 5 February 2011 (EST)
2 days after transfection
--Paul.xu 10:33, 6 February 2011 (EST)
3 days after transfection
--Paul.xu 09:09, 7 February 2011 (EST)
4 days after transfection
--Paul.xu 09:52, 8 February 2011 (EST)
| well number | transfection reagent | amount of TR being used (ul) | DNA(PSMPUW-CMV-EGFP) (ug) |
| 1 | LPEI25 | 1 | 1 |
| 2 | LPEI25 | 3 | 1 |
| 3 | BPEI25 | 1 | 1 |
| 4 | BPEI25 | 3 | 1 |
| 5 | PEIMAX | 1 | 1 |
| 6 | PEIMAX | 3 | 1 |
| 7 | PolyExpress | 3 | 1 |
| 8 | GeneExpresso | 3 | 1 |
| 9 | GeneExpresso Plus | 3 | 1 |
| 10 | Proteinone | 2 | 1 |
| 11 | Proteinone | 4 | 1 |
| 12 | TurboFect | 3 | 1 |
1) Add transfection reagent to 50 ul DMEM
2) Add DNA to 50 ul DMEM
3) Add transfection reagent in step 1) to DNA (step 2), mix well and keep for 20 min at room temperature
4) Add the mixture in step 3 to cells in 12 well plate.
--Ztao 15:03, 4 February 2011 (EST)