Transfection reagents comparison 6

293F cells

TR3 & TR6

dilute TR3 and TR6 in 10 mM Hepes, pH7.1, 10 mM NaCl, 1 ug DNA, 3 ul transfection reagent. 100 ul dilution

1: TR3 1:10

2. TR3 1:20

3. TR3 1:30

4. TR3 1:40

5. TR3 1:50

6. TR3 1:100

7. TR6 1:10

8. TR6 1:20

9. TR6 1:30

10. TR6 1:40

11. TR6 1:50

12. TR6 1:100

--Paul.xu 17:48, 5 April 2011 (EST)

293F cells

dilute TR3  in 10 mM Hepes, pH7.1, 10 mM NaCl, 3 ul, 1 ug DNA, 50 ul dilution vol
1. GeneExpresso

2. PolyExpress

3. PEI

4. TR3 1:10

5. TR3 1:20

6. TR3 1:30

7. TR3 1:40

8. TR3 1:50

9. TransIT-LT

10. Effectene

11. X-tremeGene 9

12 X-tremeGene HP

Cell death TR3 1:10 to 1:50 --Paul.xu 17:48, 5 April 2011 (EST)