Tris Buffer

Use this link to calculate Tris Buffer

http://www.cytographica.com/lab/HHTris.html

or

Trizma Buffer Table – pH vs. Temperature

pH at Temperature			gL for 1 M Tris-HCl		g/L for 0.05 M Solution
5 °C	25 °C	37 °C	Trizma HCl	Trizma Base	Trizma HCL	Trizma Base
7.76	7.20	6.91			7.02	0.67
7.89	7.30	7.02			6.85	0.80
7.97	7.40	7.12			6.61	0.97
8.07	7.50	7.22	127 g	23.6 g	6.35	1.18
8.18	7.60	7.30			6.06	1.39
8.26	7.70	7.40			5.72	1.66
8.37	7.80	7.52			5.32	1.97
8.48	7.90	7.62			4.88	2.30
8.58	8.00	7.71			4.44	2.65
8.68	8.10	7.80			4.02	2.97
8.78	8.20	7.91			3.54	3.34
8.88	8.30	8.01			3.07	3.70
8.98	8.40	8.10			2.64	4.03
9.09	8.50	8.22			2.21	4.36
9.18	8.60	8.31			1.83	4.65
9.28	8.70	8.42			1.50	4.90
9.36	8.80	8.51			1.23	5.13
9.47	8.90	8.62			0.96	5.32
9.56	9.00	8.70			0.76	5.47

0.2 M TrisMa(24.2 g/L)	0.2 M HCl	pH after dilution to 200 ml
50 ml	5.0 ml,  or 86 ul of 12 N HCl	9.0
50 ml	8.1 ml, or  139  ul of 12 N HCl	8.8
50 ml	12.2 ml, or  209  ul of 12 N HCl	8.6
50 ml	16.5 ml, or  283 ul of 12 N HCl	8.4
50 ml	21.9 ml, or  376  ul of 12 N HCl	8.2
50 ml	26.8 ml, or  460 ul of 12 N HCl	8.0
50 ml	32.5 ml, or  558 ul of 12 N HCl	7.8
50 ml	38.4 ml, or  659 ul of 12 N HCl	7.6
50 ml	41.4 ml, or   711 ul of 12 N HCl	7.4
50 ml	44.2 ml, or   759 ul of 12 N HCl	7.2

0.1 M Tris Buffer pH	Tris-HCl,   (mw: 157.6 g/mol)	Tris Base, Trizma Base (mw: 121.14 g/mol)
7.2	14.77	0.77
7.3	14.0	1.36
7.4	13.23	1.95
7.5	12.47	2.53
7.6	11.7	3.12
7.7	10.94	3.71
7.8	10.17	4.29
7.9	9.41	4.88
8.0	8.65	5.47
8.1	7.88	6.05
8.2	7.12	6.64
8.3	6.36	7.23
8.4	5.59	7.82
8.5	4.83	8.40
8.6	4.07	8.99
8.7	3.30	9.58
8.8	2.54	10.16
8.9	1.78	10.75
9.0	1.01	11.34

https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0

1 M TRIS pH 7.5 (1 L):  TRIS HCl 127 g  + TRIS Base 23.6 g

1 M TrisMa, Dissolve 121.14 g of Tris (Tris Base) in 750 ml H2O, adjust pH to at 25 oC	Concentrated HCl, ml
7.0	77
7.1	75
7.2	73
7.3	72
7.4	70
7.5	67
7.6	64
7.7	61
7.8	57
7.9	53
8.0	49
8.1	45
8.2	41
8.3	37
8.4	32
8.5	28
8.6	25
8.7	21
8.8	18
8.9	15
9.0	12

0.1 M Tris-HCl (12.1g trisma in 1 L final vol), Final pH at 25°C	0.1 N HCl
7.2	89.4mL
7.3	86.8
7.4	84.0
7.5	80.6
7.6	77.0
7.7	73.2
7.8	69.0m
7.9	64.0
8.0	58.4
8.1	52.4
8.2	45.8
8.3	39.8
8.4	34.4
8.5	29.4
8.6	24.8
8.7	20.6
8.8	17.0
8.9	14.0

12.472.53

11.73.12

10.943.71

10.174.29

9.414.88

8.655.47

7.886.05

7.126.64

6.367.23

5.597.82

4.838.40

4.078.99

3.309.58

1.7810.75

1.0111.34