Trouble report
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| # | Name | Antibiotics | Vector | Enzymes | Status | Date | possible reasons for failure | Solution or remedy |
| Subclone | ||||||||
| 931 | pT7A3-PAK16D ligase |
K | 931-1(927 as vector) | Nde1+HindIII | doing | the vector has two bands after 1 hour digest, might contaminated? | digest with fastdigest 1 hour | |
| PCR cloning | ||||||||
| 3206 | pEF1-HTLV-LAMP-1 |
Amp | 3165 | BamH1+Not1 | miniprep digestion no insert | 7/31/18 |
no insert | |
| 998 | pT7A3-6His-NLS-dCas9-NLS-6xHis |
K | 1708 | HindIII+Xho1 | 7/31/18 |
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| 3235 | pCMV5-IL2-MICA-(3c)-Fc-WPRE-OriP |
Amp | 3237 or 3232 | Nhe1+Kpn1 | just start to do |