Use CPP to Promote Transduction

Use CPP to promote Transduction of Tat-Cre and Tat-Flp

1. Transfect 293T cells with reporters,

6 wells plate, 3 ug DNA each well

Cre: 2181 pCMV-LoxP-kozak-TurboRFP-ODC-2Stops-LoxP-TurboGFP

Flp: 2013 pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP

2. Split cells next day into 12 or 24 wells plate

3. Add 0.5 μM Tat-Cre or Tat-Flp

and add the following peptides at 5 μM, make sure there are negative controls (No tat-Cre/Tat-Flp, No peptide)

Use this page to calculate MW to concentration

=============
R9-HA
RRRRRRRRRGLFEAIEGFIENGWEGMIDGWYGC
MW: 4100

1 mg-->243.9 nmole, 1 mg in 1 ml--> 243.9 μM

=============
Tat-HA2
RRRQRRKKRGGDIMGEWGNEIFGAIAGFLGC
MW: 3536

1 mg-->285.7 nmole, 1 mg in 1 ml--> μM
=============
HA2-R9
GDIMGEWGNEIFGAIAGFLGGGRRRRRRRRRGC
MW: 3735

1 mg-->270 nmole, 1 mg in 1 ml--> 270 μM
=============
Tat-IFN7
RRRQRRKKRGLFEAIEGFIENGWEGMIDGWYGC
MW: 4016

1 mg-->250 nmole, 1 mg in 1 ml--> 250μM

4. Incubate o/n and take a picture of cells. Compare transduction efficiency.

Navigation menu