Yidan 2016 9 28
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EXP1
[edit]Purpose: PCR of 2489
Procedure:
Template: Trec-combo
Primer: 2477-ALB-NdeI-F, 2478-betaActin-XhoI-R
PCR cycle:
1 min at 94 oC
30 cycles:
30 s at 94 oC
30 s at 55 oC
1.5 min at 72 oC
5 min at 72 oC
Result:
EXP2
[edit]Purpose: PCR of maize actin
Procedure:
Template: Maize genomic DNA
Primer: 2482-maize-Actin1-Nde1-F, 2482-maize-Actin1-Xho1-R
PCR cycle:
1 min at 94 oC
30 cycles:
30 s at 94 oC
30 s at 55 oC
30 s at 72 oC
5 min at 72 oC
Result:
